RNA

Part:BBa_K5055007:Design

Designed by: Laurine Wachspress   Group: iGEM24_Ionis-Paris   (2024-10-01)


small hairpin RNA RdRp BYV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 195
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 196
    Illegal PstI site found at 210
    Illegal NotI site found at 7
    Illegal NotI site found at 203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 196
    Illegal PstI site found at 210
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

At the end of the sequence, we added the Origin of Assembly Sequence of the TMV. This will allow the shRNA to be encapsulated in the viral capsid of the TMV.


Source

This part corresponds to a fragment of the RNA polymerase sequence of Beet Yellows Virus. Its sequence was found on NCBI.

References